Report for Office of the Chief of Naval Research Contract N00014-92-J- 1695
Abstract
Pharmacological approaches to the optimization of the oxygen affinity of liposome-encapsulated hemoglobin (LEH), a potential blood replacement fluid, have been studied. In our work we have researched the potential utility of LR16, L35, and related analogues in optimizing the oxygen affinity of LEH. Twenty drugs were synthesized and tested for their ability to modulate the P50 value of purified human hemoglobin. A new agent, 2-4-(3,5-2-methylpropionic acid (compound 02-50), was found to display activity greater than that of LR16, and approximately equivalent to the activity displayed by L35. At 02-50 concentrations of 0.75 mM and 1.5 mM, respectively, the P50 values of LEH preparations containing human hemoglobin stripped of 2,3-diphosphoglycerate were increased from 10 mm Hg to P50 values of 32 mm Hg and 61 mm Hg, respectively.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1993
- Accession Number
- ADA270451
Entities
People
- Thomas G. Burke
Organizations
- Ohio State University