Characterization of the Sulfur Mustard Vapor Induced Cutaneous Lesions on Hairless Guinea Pigs
Abstract
The identification of antivesicant compounds has been hindered by the lack of a suitable in vivo model. Our laboratory has been evaluating the hairless guinea pig as a useful animal model to mimic the human cutaneous response to sulfur mustard (HD) exposure. The characterization of two cutaneous responses to HD, quantification of erythema using a reflectance color meter, and pathology by light microscopy, is described in this manuscript. Fifty-two hairless guinea pigs were exposed to saturated HD vapor using a vapor cup technique. The dose response of HD vapor was established by varying the duration (2-11 min) of skin exposure to an HD vapor concentration of approx. 0.77 g/m 3 under occluded caps. The degree of erythema was determined by measuring the increase in the reflectance meter's a* (relative parameter measuring the degree of redness) response for each exposure site at 4, 5, 6, and 24-h postexposure. A linear increase in erythema was observed between the 2- and 4-min HD exposure doses for each postHD observation. The increase in erythema reached a maximum with the 5-min HD exposure dose. H D exposures between 5 and 11 min produced the same degree of erythema.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1992
- Accession Number
- ADA270784
Entities
People
- Catherine R. Bangledorf
- Edward T. Clayson
- Ernest H. Braue Jr.
- Irwin Koplovitz
- Larry W. Mitcheltree
- Marty R. Litchfield
Organizations
- United States Army Medical Research Institute of Chemical Defense