Monoclonal Antibody Detection of Chlorinated Benzenes on Contaminated Sediments
Abstract
The modified soil ELISA, which eliminates the need for contaminant extraction prior to analysis, was demonstrated using crushed brick as a model soil matrix. Preliminary data were collected which tested for 1,2 - dichlorobenzene and 4-chloroaniline. Monoclonal antibodies were produced to three chlorobenzenes (chlorobenzene, 1,2-dichlorobenzene, 1,3-dichlorobenzene). Cross-reactivity experiments conducted with the antibodies to 4-chloroaniline showed strong differentiation between the target analyte and closely related chlorinated benzyl compounds. The antibodies to the chlorobenzenes continue to be used in developing a nonextractive soil immunoassay for chlorobenzenes.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 27, 1993
- Accession Number
- ADA270824
Entities
People
- Deborah J. Mossman
- Thomas L. Feldbush