Identification, Purification and Characterization of Major Antigenic Proteins of Campylobacter jejuni
Abstract
Evidence from developing countries and volunteer studies indicates that immunity to Campylobacter jejuni and Campylobacter coli maybe acquired, but the antigenic basis for this protection is poorly defined. We have purified to homogeneity four proteins with molecular weights of 28,000 (PEB1), 29,000 (PEB2) , 30,000 (PEB3), and 31,000 (PEB4) from epidemic C. jejuni strain 81-176 using acid extraction and sequential ion-exchange, hydrophobic interaction, and gel filtration chromatography. The relative amino acid compositions of these four proteins are similar NH2-terminal sequence analysis indicates that all four proteins are different, although the first 35 amino acids of PEB2 and PEB3 are 51.4% homologous.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1991
- Accession Number
- ADA271905
Entities
People
- Martin J. Blaser
- Richard T. Ellison Iii
- Zhiheng Pei
Organizations
- Veterans Administration Medical Center