Isolation and Characterization of Two Campylobacter Glycine-Extracted Proteins That Bind to HeLa Cell Membranes

Abstract

Two immunogenic proteins of 27 (CBF1) and 29 (CBF2) kDa from enteropathogenic Campylobacter species appear to bind to mammalian cells. We purified these two proteins from a pathogenic and adherent Campylobacter jejuni strain to homogeneity using acid extraction, preparative gel electrophoresis and electroelution. Polyclonal rabbit antisera to these proteins were prepared. Immunologic studies indicate that CBF1 corresponds to the PEB1 and CBF2 corresponds to the PEB4 described by Pei et al.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1993
Accession Number
ADA271906

Entities

People

  • Chislaine Grollier
  • Jean-marie Pages
  • Martin J. Blaser
  • Michele Kervella
  • Zhiheng Pei

Organizations

  • Veterans Administration Medical Center

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Adhesion
  • Albumins
  • Anti-Bacterial Agents
  • Antibodies
  • Antigens
  • Bacteria
  • Biomedical And Dental Materials
  • Cell Membrane
  • Cells
  • Cellular Structures
  • Chemistry
  • Epithelial Cells
  • Gel Electrophoresis
  • Immune Serums
  • Microbiology
  • Polymeric Films
  • Proteomics

Fields of Study

  • Biology

Readers

  • Computer Engineering
  • Immunology
  • Microbial Pathology