A Sensitive Method for the Detection of Immune Complexes in Human Gingival Crevicular Fluid

Abstract

An assay for immune complexes (ICs) in gingival crevicular fluid (GCF) could be potentially useful to ascertain the risk of periodontal disease activity at specific oral sites. This paper describes an ELISA-based method sufficiently sensitive to measure ICs in GCF samples at levels below 1 Ag/ml. Static GCF samples taken from 5 adults showed IC levels ranging from 5 to Host response, Immune complexes, Gingival crevicular fluid, ELISA 166 microgram/ml. Additional tests of 10 GCF samples from 1 adult were conducted with complement components and indicated that either Clq or rabbit anti-human C3d was suitable as a capture agent in the assay procedure. Further application of this assay may help to assess the usefulness of IC levels in GCF samples as possible diagnostic indicators of periodontal disease activity.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1993
Accession Number
ADA272236

Entities

People

  • B. L. Lamberts
  • D. W. Turner
  • E. D. Pederson

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Fields of Study

  • Medicine

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