Hydrogen-Rated System for In Vitro Studies at Pressure: Experimental Validation

Abstract

A special hyperbaric perfusion apparatus was constructed to study the neurophysiologic effect of high pressure and the pharmacology of various gaseous agents using the isolated nerve terminal (synaptosome) tissue preparation. This study was performed to validate the performance of this apparatus in comparison with an alternate device constructed of different materials. This was motivated by theoretical considerations and some anecdotal indications that stainless steel, utilized in the fabrication of the hyperbaric device, might possibly be toxic to synaptosomes. Time course of K+-stimulated release of 3 H gaba from guinea pig cerebrocortical synaptosomes was the same for the stainless steel hyperbaric superfusion device as for an apparatus made of plastic. As a practical matter, stainless steel construction material does not appear to affect synaptosome function.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1993
Accession Number
ADA273487

Entities

People

  • G. Imbert
  • H. J. Moore
  • J. S. Colton
  • Katherine E. Miller

Organizations

  • Naval Medical Research Center

Tags

DTIC Thesaurus Topics

  • Analysis Of Variance
  • Animals
  • Biomedical Research
  • Central Nervous System
  • Chemistry
  • Construction
  • Construction Materials
  • Data Analysis
  • Experimental Data
  • Fatty Acids
  • High Pressure
  • Hydrogen
  • Laboratory Animals
  • Navy
  • Nervous System
  • Rodents
  • Time Intervals

Readers

  • Marine Mammal Biology
  • Metallurgy
  • Neuroscience