AFRRI Reports, Third Quarter 1993
Abstract
The adherence of undifferentiated 51 Cr-labeled HL-60 (0.5 x 106 HL- 60 cells/well) cells was monitored on influenza virus-infected HUVEC monolayers. Whereas only 3.0 + or - 1.6% (n = 36) of HL-60 cells adhered to uninfected HUVEC, adherence was increased to 41.7 + or - 2.2% (n = 6), 79.7 + or - 1.2% (n = 6), 83.9 + or - 0.7% (n = 6), and 84.4 + or - 0.5% (n = 6) on HUVEC infected for 7 h at a MOI of 1,3,6, and 9, respectively. In comparison, HL-60 cell adherence increased to 35% when HUVEC monolayers were stimulated with LPS (0.2- 20 micrograms) for 4 h. Increased adherence to infected HUVEC occurred at 5 h postinfection, peaked at 7 h, and was maintained at 24 h postinfection. Active virus and metabolically active endothelial cells were required to mediate the virus-induced adherence. E-selection and ICAM-1 Ag were upregulated 78.3- and 4. 1-fold, respectively, by LPS (0.02-20 micrograms, 4h) whereas virus infection (7 h) only increased these proteins 2.6- and 1.4-fold with a MOI greater than or equal 16.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1993
- Accession Number
- ADA273787
Entities
Organizations
- Armed Forces Radiobiology Research Institute