Antifouling Agents from Eelgrass: Identification, Characterization and Mode of Action

Abstract

This research has focused on the isolation and structural characterization of natural, non-toxic antifoulants produced by the eelgrass Zostera marina L. Chemical purification of a single active agent from fractions produced by aqueous-organic phase partitioning, size filtration chromatography and HPLC was directed by microbial attachment assays. Structural characterization of the isolated agent was accomplished by high resolution nuclear magnetic resonance and mass spectrometry. The isolated compound and several analogs were synthesized. Laboratory bioassays and short term (<10 day) field experiments indicate that the natural agent and at least of the one synthetic analogs show significant antifouling activity against marine bacteria, algal spores, benthic diatoms, tube worms and barnacles. Evaluation of their antifouling potential continues. A patent application describing the use of these compounds as non-toxic antifouling agents is in preparation.

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Document Details

Document Type
Technical Report
Publication Date
Dec 03, 1993
Accession Number
ADA274070

Entities

People

  • Hewson Swift
  • Richard C. Zimmerman

Organizations

  • University of Chicago

Tags

DTIC Thesaurus Topics

  • Air Force
  • Antifouling
  • Attachment
  • Bacteria
  • Barnacles
  • Biological Sciences
  • Biology
  • Cell Biology
  • Fouling Organisms
  • High Resolution
  • Liquid Chromatography
  • Magnetic Resonance
  • Mass Spectrometry
  • Military Research
  • Molecular Biology
  • Nuclear Magnetic Resonance
  • Patent Applications

Fields of Study

  • Environmental science

Readers

  • Analytical Chemistry
  • Aquatic Ecology
  • Organic Chemistry

Technology Areas

  • Biotechnology