Fish as a Predictive Model for Epigenetic Carcinogens
Abstract
This study extends the in vivo and in vitro evaluation of peroxisome proliferators in rainbow trout and medaka. In vitro studies with primary cultures of rainbow trout and medaka hepatocytes showed these species to be weak responders to peroxisome proliferators as measured by increased fatty acyl-CoA oxidase activity. Studies conducted in vitro also showed that the peroxisome proliferating agents displayed relatively low capacity to induce a mitogenic response as measured by tritiated thymidine uptake in primary cultures of rainbow trout and medaka hepatocytes. Similar results were shown with rainbow trout in vivo in which no statistically significant increases in liver-to-body weight ratio nor hepatic DNA content were observed following exposure to a known rodent liver mitogen (lead). Hepatic ornithine decarboxylase (ODC) levels were measured in trout and individual medaka and the activity was shown to be 100- 200-fold higher in these fish models compared to the rodent. In addition, cell- to-cell communication was characterized by dye-coupling for the first time in vitro with rainbow trout hepatocytes and was shown to be adversely affected by the known tumor promoter TPA. A Project Summary is also included. Toxicity, Fish, Carcinogen, RAIII, Lab animals, Peroxisomes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 23, 1993
- Accession Number
- ADA274432
Entities
People
- Edward Calabrese
- Paul T. Kostecki
Organizations
- University of Massachusetts Amherst