DNA Lesions in Medaka (o. latipes): Development of a Micro-Method for Tissue Analysis Using Gas Chromatography-Mass Spectrometry

Abstract

We have reached a point where substantial reductions have been made in the amount of tissue required for GC-MS analysis. In the future, we will analyze the tissues from a number of normal and exposed organisms using the micro method to hopefully validate the approach. The work on monoclonal antibodies is proceeding favorably. Shortly, we expect to obtain a sufficiently pure 8-OH-adenosine to initiate the antigen phase of the work. The FT-IR effort, if successful, will allow for a rapid assessment of DNA modifications in relation to carcinogenesis using as little as a few micrograms of DNA. Overall, the project is on schedule. Fish, Molecular biology, Environmental assessment, RA III.

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Document Details

Document Type
Technical Report
Publication Date
Jul 29, 1993
Accession Number
ADA275291

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  • Donald C. Malins

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DTIC Thesaurus Topics

  • Breast Cancer
  • Carcinoma
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Formic Acid
  • Gas Chromatography
  • Hydroxyl Radical
  • Mass Spectra
  • Mass Spectrometry
  • Molecules
  • Neoplasms
  • Organic Chemistry
  • Phase
  • Spectra
  • Spectrometry
  • Statistical Analysis

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  • Analytical Chemistry
  • Molecular and genetic basis of cancer.
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