Evaluation of Buprenorphine Hydrochloride and Butorphanol Tartrate on the Inflammatory Reaction of the Sereny Test
Abstract
Two blinded studies were conducted to evaluate the effects of selected systemic analgesics on the Sereny Test in outbred Hartley guinea pigs. Study 1 evaluated the recommended dosages for two systemic analgesics; study groups consisted of those receiving butorphanol tartrate (n=16), those receiving buprenorphine hydrochloride (n=16), and untreated controls (n=5). Study 2 evaluated a low-dose buprenorphine hydrochloride group (n=16) and an untreated control group (n=5). All animals were inoculated with Shigella flexneri, strain 2a 2457T, onto the cornia and conjunctiva of each eye. At the onset of clinical signs, analgesics were administered to test groups. The degree of keratoconjunctivitis was evaluated per standard procedure; animals were weighed daily. After 7 days, animals were euthanatized and the eyes were removed for histologic morphometric evaluation. Clinical observations of keratoconjunctivitis in both studies were not significantly different. Histologic morphometry confirmed clinical observations when each analgesic treatment group was compared with the corresponding untreated control group. Although heavy buildup of periorbital mucopurulent discharge in the buprenorphine study-1 group complicated clinical observations, the lower dose of buprenorphine (study-2) appears compatible for use. Sereny test, Analgesics, Guinea pigs, Inflammatory response.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1993
- Accession Number
- ADA275302
Entities
People
- James R. Swearengen
- Judith A. Davis
- Peter J. Weina
- Rebecca A. Cockman-thomas
Organizations
- Walter Reed Army Institute of Research