Preclinical Investigation of Lyophilized Platelet Preparations
Abstract
The characterization of rehydrated human and canine platelets is underway. We have begun the characterization of rehydrated platelets using SDS-PAGE and immunoblotting of human and canine platelet preparations. Rehydrated and fresh platelets were lysed using a freeze-thaw protocol or using a detergent such as Triton X-100. We have found that, as expected, the rehydrated platelets are not lysed with the facility of fresh platelets. SDS-PAGE was performed on the platelet lysates and silver staining was used to visualize the proteins. Our results suggest that rehydrated human platelet lysates have a similar but nonidentical protein profile on SDS-PAGE when compared to fresh human platelet lysates. Similar studies are currently underway using canine fresh and rehydrated platelets.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 02, 1994
- Accession Number
- ADA275899
Entities
People
- Arthur P. Bode
Organizations
- East Carolina University