Genetic Methods for Rapid Gene Localization in Hyperthermophilic Eubacteria

Abstract

We initiated investigations along several lines to develop genetic methods of analyses with Thermotoga neapolitana. (1) We investigated the conditions necessary for optimal growth of T. neapolitana on solid media under anaerobic conditions. (2) We attempted to isolate mutants to provide genetic markers for the development of gene transfer methods. (3) We explored a number of methods to introduce DNA into T. neapolitana. (4) A cryptic Thermotoga plasmid was discovered and its potential as a cloning vector was investigated. (5) A cosmid library and large chromosomal fragments will be used to located and map genes.

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Document Details

Document Type
Technical Report
Publication Date
Aug 31, 1993
Accession Number
ADA276062

Entities

People

  • Kenneth M. Knoll

Organizations

  • University of Connecticut

Tags

Communities of Interest

  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Bacteria
  • Biological Processes
  • Biology
  • Biomes
  • Electrophoresis
  • Environment
  • Frequency
  • Gel Electrophoresis
  • Genetic Markers
  • Genetics
  • Microbial Genetics
  • Microbiology
  • Microbiomes
  • Microorganisms
  • Mountains
  • Prokaryotes

Fields of Study

  • Biology

Readers

  • Molecular Genetics

Technology Areas

  • Biotechnology