Lectin Enzyme Assay Detection of Viruses, Tissue Culture, and a Mycotoxin Simulant
Abstract
Nonspecific lectin detection of virus groups depends on the lectin binding with the specific virus protein polysaccharide. Prevot and Guesdon used wheat germ agglutinin (WGA) to detect rotavirus antigen in an erythro-lectin immunoassay. WGA has an avidity specificity for N-acetyl glucosamine and a lesser specificity for sialic acid. Delagneau, Perrin, and Atanasiu found that the following five different lectins had an affinity for rabies virus: Phaseolus vulgaris (PHA)- red kidney bean agglutinin, Triticum vulgaris (WGA)- wheat germ agglutinin, Ricinus culinaris (RCA)-castor bean agglutinin, Lens culinaris (LCA) - lentil agglutinin, Concanavalia ensiformis (Con A) - jack bean agglutinin (to a lesser degree)
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1988
- Accession Number
- ADA276469
Entities
People
- William H. Kraybill
Organizations
- Edgewood Chemical Biological Center