Comparison of the Effects of Hyperbaric Oxygenation and Transforming Growth Factor Beta on Wound Healing in Rats
Abstract
A pilot investigation was conducted to determine the effects of hyperbaric oxygenation (HBO) and topical transforming growth factor beta (TGF- Beta 1), alone and in combination, on the healing of standardized excisional wounds in normal rats. Excisional wounds were made on the dorsum of 96 adult male Sprague-Dawley rats, and rats were randomly allocated into groups receiving daily topical TGF-Beta 1 or placebo solution. Groups were subdivided into animals treated with either ground level air, 2.0 ATA air, ground level 100% oxygen, or 2.0 ATA 100% oxygen. Each 4 days, wounds were measured, animals weighed, and 2 animals euthanized for histologic study to assess healing. The study found that TGF-Beta I treated rats healed faster, with maximum effect 12 days after wounding. Breathing mix and depth had no significant effect on healing. Animals treated with oxygen gained significantly more weight than animals treated with air.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1994
- Accession Number
- ADA277751
Entities
People
- Carney B. Jackson
- Daniel T. Fitzpatrick
- Douglas C. Warren
- Frederick W. Rudge
- John W. Fanton
Organizations
- United States Air Force School of Aerospace Medicine