DNA Polymorphism in the Genome: A Raman Spectroscopic Study

Abstract

A laser Raman microscope was used to obtain spectra from polytene chromosomes in transcriptionally active salivary gland nuclei of Drosophila melanogaster third-instar larvae. Bands for DNA, nucleosomes, proteins, and nuclear membrane fatty acids were identified in difference spectra generated from 1:1 subtraction of the cytoplasm spectra from nuclear spectra. The presence of bands corresponding to B-, C- and disordered-DNA secondary structure indicates that DNA polymorphism exists within salivary gland cells of D. melanogaster.

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Document Details

Document Type
Technical Report
Publication Date
Mar 17, 1994
Accession Number
ADA277764

Entities

People

  • J. W. Powell

Organizations

  • Reed College

Tags

DTIC Thesaurus Topics

  • Archaeal Proteins
  • Bacteriorhodopsin
  • Cells
  • Chemistry
  • Chromosomes
  • Crystal Lattices
  • Cytoplasm
  • Diffraction
  • Dynamics
  • Eukaryotes
  • Lattice Dynamics
  • Molecules
  • Raman Spectroscopy
  • Salivary Glands
  • Scattering
  • Spectra
  • Spectroscopy

Fields of Study

  • Biology
  • Physics

Readers

  • Molecular Biology and Genetics
  • Molecular Genetics
  • Optical Physics and Photonics.

Technology Areas

  • Directed Energy