Molecular Ecology of Bacterial Populations in Environmental Hazardous Chemical Control

Abstract

The major outcomes of the current work are: (1) Development of a new molecular strategy, mRNA extraction from soil, assesses the catabolic activity of soil bacteria in situ. (2) Quantitative the association between the biosensor bioluminescence response and the PAHs bioavailability present in the waste environment. (3) Demonstration the ability of NAH plasmid to mediate the initial biodegradation reactions in the catabolic pathway of fluorence. The current research work is focuses on developing new molecular diagnostics' method for measuring in situ PAH biodegradation activity and co-related the bioluminescence response, that produced by a naphthalene-lux reporter strain, to the bioavailability of different pollutants in the real environment. In addition, catabolism of a tricyclic aromatic hydrocarbon, fluorene, mediates by a NAH plasmid is also investigated.

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Document Details

Document Type
Technical Report
Publication Date
Jan 14, 1994
Accession Number
ADA278340

Entities

People

  • Gary S. Sayler

Organizations

  • University of Tennessee

Tags

Communities of Interest

  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Aromatic Hydrocarbons
  • Aromatic Polycyclic Hydrocarbons
  • Biodegradation
  • Biosensors
  • Chemical Synthesis
  • Chemistry
  • Cyclic Hydrocarbons
  • Environment
  • Environmental Monitoring
  • Environmental Protection
  • Environmental Restoration And Remediation
  • Fluorenes
  • Hydrocarbons
  • Mrna
  • Naphthalenes
  • Organic Chemistry
  • Pseudomonas Infections

Fields of Study

  • Biology
  • Environmental science

Readers

  • Environmental Engineering.
  • Molecular Genetics
  • Organic Chemistry

Technology Areas

  • Biotechnology
  • Biotechnology - Bioremediation