Analysis of Saxitoxin from Urine Using Continuous-Flow Fast Atom Bombardment & Electrospray Ionization Mass Spectrometry
Abstract
A method for analysis of saxitoxin (STX) in urine of rats treated with STX was developed using CBA columns for clean-up of the extract and hydrogen peroxide oxidation prior to analysis by HPLC. Detection of STX is linear between 0.1 and 5 ng when measured by either peak height or area. It was used for analysis of urine from rats treated with 1 microgram total STX. The limit of detection of pure standard is 100 pg. Saxitoxin was found in the urine of the treated rat (I pg total dose) as soon as 10 min after treatment and up to 8 hrs after dosing. In general, the excretion pattern was bimodal , i e. , the largest amount was found 1 hr after treatment, followed by a decline 2 and 3 hrs afterward, and then a reappearance of larger amounts at 6 and 8 hrs. Adult female rats tolerate a 1 pg dose of STX given by intraperitoneal injection but succumb to 3 to 4 pg (i.p.) within 3 min.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 30, 1993
- Accession Number
- ADA278874
Entities
People
- Chester J. Mirocha
Organizations
- University of Minnesota