Dengue Type-2 Virus Envelope Protein Made Using Recombinant Baculovirus Protects Mice Against Virus Challenge
Abstract
The gene coding for the envelope (E) glycoprotein of dengue 2 virus was cloned into baculovirus (Autographa californica nuclear polyhedrosis virus, AcNPV). The recombinant virus contained the entire E protein gene, preceded by 38 nucleotides from the end of the prM gene and followed by the first 83 nucleotides of NS1. When expressed in Spodoptera frugiperda (Sf9) cells, about 1 mg of recombinant E antigen was made per 10/9/ cells. This antigen reacted with polyclonal, anti-dengue type 2 antibody and a dengue type 2-specific, neutralizing monoclonal antibody. Balb/c mice immunized with the recombinant antigen produced only non-neutralizing antibody against dengue 2 virus but were partially protected against morbidity and mortality after intracranial challenge with virulent dengue 2 virus. Dengue-2 Virus, E Protein, Baculovirus.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1994
- Accession Number
- ADA279844
Entities
People
- Jeanne Burrous
- Robert Feighny
- Robert Putnak
Organizations
- Walter Reed Army Institute of Research