Effect of Hyperbaric Carbon Dioxide on Spores and Vegetative Cells of Bacillus stearothermophilus
Abstract
Spore suspensions in water, 0.05M pH 7 phosphate buffer, 0.05M pH 4.5 and 0.05M pH 4.0 acetate buffer, and antibiotic assay media supplement broth (AAMS) were subjected to 50 psi to 1100 psi carbon dioxide at various temperatures in Parr bombs for up to 96 hours. Spores were resistant to high pressure carbon dioxide treatment at each pH and at both high and low temperatures. Spore suspensions that were first heat activated and then incubated at 550C for a short time increased sensitivity to destruction by CO2. Although spores were resistant to destruction by hyperboric CO2 low pressure (50 psi) CO2 was seen to completely inhibit spore germination and outgrowth. Inhibition to germination was found to be reversible. Spore germination was found to be impaired in carbonated AAMS broth. However, the addition of 50 mM naHCO3 to AAMS enhanced germination and outgrowth. Vegetative cells, Bacillus stearothermophilus, Carbon dioxide, Thermophilic bacteria, Spores, Thermophilic Spoilage, Shelf life, Perishable commodities.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1994
- Accession Number
- ADA280115
Entities
People
- Anthony Sikes
- Chester T. Roskey
Organizations
- United States Army Soldier Systems Center