Production of Antigens and Antibodies for Diagnosis of Arbovirus Diseases

Abstract

Arbovirus antigens and antibodies were produced and standardized for use in ELISA. Antigens were produced by sucrose-acetone extraction of suckling mouse brain for 25 arboviruses and residual infectivity was inactivated with beta-propiolactone. An additional 8 viruses were passaged in mice and the mice were stored frozen awaiting sucrose-acetone extraction of the brains. Rabbits were immunized successfully intravenously with 9 arboviral immunogens grown in RK-13 rabbit cells. Testing of the resulting ammonium sulfate precipitated IgG by ELISA showed generally excellent titers. The testing is continuing.

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Document Details

Document Type
Technical Report
Publication Date
May 20, 1994
Accession Number
ADA280116

Entities

People

  • Robert E. Shope

Organizations

  • Yale University

Tags

DTIC Thesaurus Topics

  • Acquisition
  • Animals
  • Antibodies
  • Arboviruses
  • Biomedical Research
  • Contracts
  • Extraction
  • Laboratory Animals
  • Lactones
  • M Band
  • New Jersey
  • Production
  • Propiolactones
  • Prototypes
  • Residuals
  • Viruses

Fields of Study

  • Biology

Readers

  • Immunology
  • Virology (or Medical Virology).