Production of Antigens and Antibodies for Diagnosis of Arbovirus Diseases.

Abstract

Antigens and antibodies were produced and standardized for use in the ELISA. Antigens were produced by sucrose-acetone extraction of suckling mouse brain for Germiston, Qalyub, Sicilian, vesicular stomatitis Indiana, and Ganjam viruses. The antigens were inactivated with beta-propiolactone. Rabbits were immunized successfully intravenously with Ross River, Germiston, and Japanese encephalitis viruses using immunogens grown in RK-13 rabbit kidney cells. These animals were boosted 2 months after the initial immunization. Titers in ELISA for optimal coating of the solid phase ranged between 1:500 and 1:64000. Antigens, Antibodies, Arbovirus, Enzyme linked immunosorbent assay

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Document Details

Document Type
Technical Report
Publication Date
May 20, 1994
Accession Number
ADA280199

Entities

People

  • Robert E. Shope

Organizations

  • Yale University

Tags

DTIC Thesaurus Topics

  • Animals
  • Antibodies
  • Arboviruses
  • Biomedical Research
  • Cells
  • Diseases And Disorders
  • Encephalitis
  • Extraction
  • Immunization
  • Laboratory Animals
  • Lactones
  • Mouth Diseases
  • Phase
  • Production
  • Propiolactones
  • Solid Phases
  • Viruses

Fields of Study

  • Biology

Readers

  • Virology (or Medical Virology).