Chemical and Structural Characterization of Nitroaromatic Adducts with Hemoglobins
Abstract
The reactivity of dinitrobenzene (ortho-, para-, meta-DNB) with bovine he has been studied in vitro using spectroscopic and chromatographic methods. Deconvolution of time-dependent VIS/UV spectra of mixtures of hemoglobin with DNB show that DNB reacts readily with deoxyhemoglobin to form methemoglobin without the need for metabolic activation that has been inferred from previous in vivo studies. No reaction is observed with oxy- or carboxy- hemo globin. Column and HPLC chromatographic separations of hemoglobin treated with 14C radiolabelled DNB did not produce evidence for adduct formation. Similar analyses of hemoglobin from radiolabelled DNB- and TNB-treated rats also showed no direct evidence of strong adducts. Dinitrobenzene, Hemoglobin, Deoxybemoglobin, Carboxyhemoglovin, Nitroaromatic
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 1994
- Accession Number
- ADA280533
Entities
People
- Walter J. Stevens
Organizations
- National Institute of Standards and Technology