Effect of Emetine on the Specific Association of T-2 Toxin with Mammalian Cells
Abstract
The effects of emetine on the association of T-2 toxin with Chinese hamster ovary cells were examined. T-2 toxin-cell association at both 4 deg C and 37 deg C was reduced by up to 90% after preincubation of cells with emetine. Emetine-induced reduction in T-2 toxin-cell association was time-, temperature-, and concentration-dependent. A 4-min preincubation with emetine at physiological temperature was required to develop the maximum inhibitory effect. After brief exposures < or = 5 min), emetine's inhibitory effects on toxin-cell association were reversible. However, after longer exposure periods to emetine (60 min), toxin-cell association was irreversiby blocked.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1993
- Accession Number
- ADA281341
Entities
People
- Dennis L. Leatherman
- John L. Middlebrook
Organizations
- United States Army Medical Research Institute of Infectious Diseases