Rapid Detection of Mycobacteria in Patients with HIV Infection
Abstract
The Polymerase Chain Reaction (PCR), along with hybridization to chemiluminescent DNA probes, was used to detect Mycobacteria potentially present in patient specimens from the Mycobacteriology Laboratory at Walter Reed Army Medical Center (WRAMC). DNA from specimens were prepared by two different methods, and used in the PCR amplifications. Primers were evaluated using both known and unknown (blinded) samples. With known samples, the genus specific primers detected 74% of Mycobacteria positive samples, while the M. tuberculosis primers were able to amplify M. Tuberculosis DNA in 59% of the positive samples. With the blinded samples, the genus specific primers were able to detect Mycobacterial sequences in 3 of 8 (37.5%) of the Mycobacteria-containing samples. There no M. tuberculosis containing specimens in the blinded samples, and all samples were negative for M. tuberculosis by PCR
Document Details
- Document Type
- Technical Report
- Publication Date
- May 16, 1994
- Accession Number
- ADA281636
Entities
People
- David C. Fritzinger
Organizations
- Armed Forces Institute of Pathology