Development of a Rapid Chair-Side Test to Detect Pathogenic Bacteria Associated with Periodontal Disease
Abstract
The purpose of this contract research was to produce a simple and rapid test that would identify dental plaque bacteria associated with early detection of periodontal disease. Spirochetes were studied because of their strong correlation with destructive, progressive periodontal disease. Monoclonal antibodies against Treponema pallidum led to the discovery of a previously unrecognized oral spirochetes in plaque at diseased sites and within gingiva near periodontal lesions. in vitro experiments demonstrated that these pathogen- related oral spirochetes (PROS) were capable of moving through living tissue; no other plaque bacteria and no cultivable oral spirochete were invasive. PROS has been found at sites of periodontal disease in HIV-negative people and in people with HIV-associated periodontal disease. Extensive efforts were made to isolate and cultivate PROS so that PROS-specific monoclonal antibodies and DNA probes could be developed for the chair-side test.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 07, 1994
- Accession Number
- ADA281927
Entities
People
- George R. Riviere
Organizations
- Oregon Health & Science University