The Role of Chemical Inhibition of Gap Junctional Intercellular Communication in Toxicology.

Abstract

Gap Junctional Intercellular Communication (GJIC) is the biological process which regulates homostatic control of cell proliferation, differentiation and adaptive functions of differentiated cells. Disruption of GJIC by toxic chemicals, either at the level of gene expression or protein function, has been correlated with teratogenesis, tumor promotion, reproductive and neurotoxicities. The mechanisms by which various epigenetic toxicants or oncogenes inhibit GJIC have been studied in this project. Modulation of phosphorylation of one gap junction protein (cx43) by two different tumor promoters (phorbol esters, DDT) has been shown to be different, yet the end result (inhibition of GJIC) is the common end point. Preliminary evidence has linked the toxic-chemical modification of the gap junction protein phosphorylation paths with altered trafficking of the protein within the cell. Further studies will extend these studies to build a solid mechanistic base for a biological risk assessment model for epigenetic or non-genotoxic chemicals.

Document Details

Document Type
Technical Report
Publication Date
Jun 14, 1994
Accession Number
ADA282452

Entities

People

  • James E. Trosko

Organizations

  • Michigan State University

Tags

DTIC Thesaurus Topics

  • Biological Processes
  • Cells
  • Gene Expression
  • Inhibition
  • Intercellular Junctions
  • Modulation
  • Phosphorylation
  • Risk
  • Risk Analysis
  • Toxicology
  • Vulnerability

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Molecular and Cellular Biochemistry
  • Rocket Propulsion.