Isolation and Preliminary Characterization of a Recombinant TAT Protein from Human Immunodeficiency Virus

Abstract

Human Immunodeficiency virus type-1 (HIV-1) Tat protein is a transactivator for viral gene expression. In order to carry out the structural and functional studies of the protein, it is necessary to isolate and purify the protein in large quantity. To produce this large quantity, the fusion protein Rop-Tat was over-expressed. The Rop-Tat is isolated from insoluble aggregates of inclusion bodies. Cyanogen bromide cleavage of Rop-Tat yields numerous protein fractions from, S-Sepharose and Q-Sepharose chromatography. Efforts are under way to determine which fractions are related to Tat. Human Immunodeficiency Virus (HIV), Transactivator protein Tat, Isolation, Recombinant DNA

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Document Details

Document Type
Technical Report
Publication Date
Jun 28, 1994
Accession Number
ADA282921

Entities

People

  • Lichun H. Walls

Organizations

  • Morgan State University

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Biomedical And Dental Materials
  • Cell Line
  • Cells
  • Chemistry
  • Cysteine
  • Elements
  • Filtration
  • Gel Electrophoresis
  • Gene Expression
  • Hiv Infections
  • Ion Exchange
  • Materials
  • Molecules
  • Polymeric Films
  • Sodium Compounds
  • Viruses

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular Genetics