In Vitro Effect of Sulfasalazine and Its Metabolites on Human T Lymphocyte Activation
Abstract
Sulfasalazine (SF) is an anti-inflammatory sulfonamide used in the treatment of rheumatoid arthritis, ulcerative colitis and ankylosing spondylitis. Its mechanism of action is not fully understood. The possible immunomodulatory role of sufasalazine and its main metabolites, sulfapyridine (SP) and 5-aminosalicylic acid (5-ASA) on T cell activation was analyzed. Peripheral blood mononuclear cells (PBMC) were separated from human blood by Ficoll-Hypaque density gradient centrifugation, depleted of monocytes by plastic adherence and further separated into B and T lymphocytes by the E-rosette method using sheep red blood cells (SRBC).
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1994
- Accession Number
- ADA283574
Entities
People
- Richard H. Mcbride
Organizations
- Air Force Institute of Technology