Identification of Staphylococcal Enterotoxin B Sequences Important for Induction of Lymphocyte Proliferation by Using Synthetic Peptide Fragments of the Toxin
Abstract
A series of 13 synthetic peptides, approximately 30 amino acids each, which spanned the entire sequence of Staphylococcal enterotoxin B, were tested to evaluate their effects on T cell proliferation in a culture system using human elutriated peripheral blood lymphocytes incubated with a specific ratio of mononuclear cells. Four peptide regions were found to inhibit SEB-induced proliferation; they included sequence 1-30 (previously thought to be involved in MHC class II binding), 61-92 (sequences which relate to the T cell receptor site), 93-112 (a linear sequence corresponding to the cysteine loop) and 130-160 (containing a highly conserved sequence KKKVTAQEL). Antisera raised to this latter peptide were capable of neutralizing SEB-induced proliferation. Antisera raised against the peptides which overlapped this sequence also were somewhat inhibitory. Neutralizing antisera were not produced from any other peptide sequence tested. In order to determine if any of these effects were non-specific with regard to SEB-induced proliferation, the peptides were tested for inhibition of phorbol dibutyryl ester-induced proliferation and only the sequence 93-112 (corresponding to the cysteinyl loop region) was consistently inhibitory (40%). Of the regions which displayed inhibition of SEB-induced proliferation, the peptide 130-160 inhibited binding of 125I-SEB to lymphocytes. These data suggest that the residues containing and surrounding the sequence KKKVTAQEL may be critical in the SEB-induced proliferation and ma be useful for developing neutralizing antisera to SEB.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1994
- Accession Number
- ADA283716
Entities
People
- Christopher Welch
- Edward Bernton
- Marti Jett
- Roger Neill
- Thomas Boyle
Organizations
- Walter Reed Army Institute of Research