Complete Protection against Plasmodium Yoelii by Adoptive Transfer of a CD8+ Cytotoxic T-Cell Recognizing Sporozoite Surface Protein 2
Abstract
BALB/c mice immunized with irradiated Plasmodium yoelii sporozoites produce antibodies and cytotoxic T lymphocytes against the circumsporozoite protein and against a 140-kDa protein, sporozoite surface protein 2 (PySSP2). Approximately 50% of mice immunized with P815 cells transfected with the gene encoding PySSP2 are protected against malaria, and this protection is reversed by in vivo depletion of CB8(+) T cells. To determine if CD8(+) T cells against PySSP2 are adequate to protect against malaria in the absence of other malaria- specific immune responses, we produced three CD8(+) T-cell clones by stimulating spleen cells from mice immunized with irradiated P. yoelii sporozoites with a mitomycin-treated P815 cell clone transfected with the PySSP2 gene. Adoptive transfer of clone TSLB7 protected 100% of mice against p. yoelli. The second clone protected 58% of mice, and the third clone provided no protection. Clone TSLB7 protected even when administered 3 h after sporozoite inoculation at a time when sporozoites had entered hepatocytes, suggesting that it is recognizing and eliminating infected hepatocytes. These studies demonstrate that cytotoxic T lymphocytes against PySSP2 can protect against P. yoelii sporozoite challenge in the absence of other parasite-specific immune responses.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1994
- Accession Number
- ADA283953
Entities
People
- Martha Sedegah
- Srisin Khusmith
- Stephen L. Hoffman
Organizations
- Naval Medical Research Center