Kinematic Analysis of Rabbit Sperm Motion in the Toxicological Assessment of Fertility
Abstract
Rabbit sperm were exposed to three concentrations each of nine compounds known or suspected to be toxic to reproductive function. Sperm motility in the presence and absence of the compounds were recorded on videotape, and the perturbations induced in sperm motions parameters by the compounds were analyzed by the CellTrak/S motion analysis system. The sperm kinematic parameters were used to derive estimates of alterations in fertility due to exposure to the compounds by the utilizing models of the reproductive risk newly developed at the University of California-Davis. The compounds were ranked into three categories of potency by taking into account the differences in the time course of action against spermatozoa and the dose response for each time point. The motion analysis provided a new objective approach to the use of sperm as biomarkers in reproductive risk assessment.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1994
- Accession Number
- ADA284306
Entities
People
- D. F. Katz
- D. H. Heitkamp
- J. Andrew
- R. J. Young
Organizations
- University of California