Molecular Approach to Hypothalamic Rhythms

Abstract

The suprachiasmatic nucleus (SCN) of the hypothalamus is the anatomical seat of the mammalian endogenous biological clock which regulates the temporal expression of hormonal and behavioral circadian rhythms. Light, serotonin and melatonin are the dominant stimuli which affect the phase of the endogenous clock. The grantee has devised strategies to identify molecules that mediate the action of these stimuli within the SCN. The grantee has identified a novel receptor for serotonin, the 5-HT7 receptor, and determined its amino acid structure. Its pharmacological ligand binding properties have been measured and a unique profile of agonists and antagonists defined. These allowed demonstration that the 5-HT7 receptor mediated circadian activity of cultured SCN. The receptor has been shown to couple to activation of adenylyl cyclase and to be synthesized by neurons of the subparaventricular zone immediately dorsal to the SCN. Molecules whose expression within the SCN is activated by light entraining cues have also been identified.

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Document Details

Document Type
Technical Report
Publication Date
Mar 14, 1994
Accession Number
ADA285164

Entities

People

  • Bruce M. Baron
  • J. G. Sutcliffe
  • Luis De Lecea
  • Mark G. Eriander
  • Patria E. Danielson
  • Timothy W. Lovenberg

Organizations

  • Scripps Research

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Brain
  • Cells
  • Chemistry
  • Chromosomes
  • Genetic Structures
  • Genetics
  • Medical Personnel
  • Neurosciences
  • Proteins
  • Sequence Analysis
  • Serotonin Agents

Fields of Study

  • Biology

Readers

  • Cardiovascular Physiology
  • Circadian Sleep-Wake Regulation and Chronobiology
  • Molecular and Cellular Biochemistry