Vasopressin Receptor Signaling and Cycling of Water Channels in Renal Epithelia.
Abstract
Water reabsorption and urinary volume regulation are important processes in maintaining normal physiological function and vasopressin (ADH) regulates water reabsorption by the kidney in response to changes in plasma volume as may occur during dehydration. This process is particularly important to soldiers who are subject to excess heat and fluid deprivation in and environments. We have been examining the mechanisms whereby ADH enhances water permeability in renal epithelia so we may find measures to enhance the kidney's responsiveness to ADH. Such regimens would maintain the water reabsorptive capacity of soldiers facing harsh conditions. Our observations suggest that an integral component of the water reabsorptive process by ADH is the cycling of water channels from the cytosol to the apical membrane. This cycling process involves exo- and endocytosis mechanisms. We have begun to examine membrane remodeling during hormone washout and a return to basal unstimulated conditions as a means to assess water channel cycling. In particular we were concerned with determining the role of protein kinase C (PKC) in this process and role of calcium mobilization. Transepithelial water flow, Vasopressin, Protein Kinase C, Endocytosis, Membrane retrieval, Filter supports for cultured cells, Cultured kidney cells
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 31, 1994
- Accession Number
- ADA285873
Entities
People
- A. J. Mia
- T. Yorio