Application of a Rapid Assay for Detection of Antibodies to Human Immunodeficiency Virus in Urine

Abstract

The use of rapid, simple test to detect antibodies to human immunodeficiency virus (HIV) in urine could be valuable for several testing situations, such as in private offices, for epidemiologic surveys, and in developing countries. The authors evaluated the performance of the SUDS HIV type 1 test to detect antibody to HIV-1 peptides in urine. Test performance and applicability of the SUDS test were compared with a routine Food and Drug Administration-licensed enzyme-linked immunosorbent assay (ELISA) and Western blot using 139 serum and urine pairs collected from autopsy cases. Using a modified procedure when testing urine by the SUDS tests, results indicated that a total of 15 serum/urine pairs were HIV-1 antibody positive by both the SUDS test and ELISA; all could be confirmed positive by Western blot.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1994
Accession Number
ADA286072

Entities

People

  • Jaya Bansal
  • Kenneth C. Hyams
  • Ling Li
  • Niel T. Constantine
  • Xiang Zhang

Organizations

  • Naval Medical Research Center

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Antigens
  • Biomedical Research
  • Biotechnology
  • Blood
  • Detection
  • Dilution
  • Diseases And Disorders
  • Health
  • Health Care
  • Health Services
  • Hiv Infections
  • Infectious Diseases
  • Medical Personnel
  • Public Health
  • United States
  • Viruses

Fields of Study

  • Biology

Readers

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  • Molecular and Cellular Biochemistry
  • Oncology and Biomarker-Based Cancer Detection.