Evaluation of Dried Storage of Platelets for Transfusion: Physiologic Integrity and Hemostatic Functionality
Abstract
The current long term dried storage study was terminated at 8 months in this reporting period. The preparations under study were (1) 0.02% permanganate in 5% BSA, and (2) 1.8% paraformaldehyde in 500 mM Trehalose stored desiccated at RT, 4 deg C, or at -70 deg C. Neither prep maintained good morphology at any temperature, and there was minimal ristocetin aggregation or hypotonic shock response remaining. At this 8 month workup, yield of intact platelets upon reconstitution was dependent on the storage conditions: 27-29% for RT, 41-46% for 4 deg C, 68-78% for -70 deg C. Our conclusion from this study reinforces what we learned from earlier storage studies: that there is significant deterioration of product at RT. In this particular experiment we found that permanganate-treated platelets, or para-platelets dried in Trehalose are as susceptible to loss of integrity over time as other preps. Our platelet handling techniques have improved since these studies were initiated. Another set of new preparations will be set aside for long-term storage in the next reporting period.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 27, 1994
- Accession Number
- ADA286078
Entities
People
- Arthur P. Bode
Organizations
- East Carolina University