Preclinical Investigation of Lyophilized Platelet Preparations
Abstract
We have previously shown that lyophilized platelets (L-Plt) retain properties of adhesion and activatability in the Baumgartner perfusion chamber (Trans 33:72S, 1993). Now we have analyzed L-Plt in two systems directly testing hemostatic function. Study Design: One is a prototype device simulating the Ivy bleeding time in vitro (IVBT) and collagen-induced thrombus formation (CITF) in recalcified whole blood (Xylum Clot Signature Analyzer: CSA); the other is an in vivo bleeding time in dogs on full clinical heart-lung bypass before and after infusion of L-Plt. Results: On the CSA, L-Plt gave an average (n=4) IVBT of 1 min 58 sec and a CITF of 73% versus 2 min 14 sec and 88% respectively for fresh platelets. Expired platelet concentrates gave indeterminate results because aggregates clogged the lines. IVBT > 6 min and CITF < 25% is typical of vWD patients. In two canine heart-lung bypass studies, the in vivo bleeding time improved from > 15 min to 5-7 min after infusion of a bolus of 2-3 x 1011 L-Plt. The corrected count increments were (1) 88% and (2) 47% based on estimated circulatory volume. Conclusions: These results demonstrate the hemostatic activity of L-Plt and their potential value in transfusion medicine.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 31, 1994
- Accession Number
- ADA286079
Entities
People
- Arthur P. Bode
Organizations
- East Carolina University