Genetic Analysis of Hyperthermophilic Archaebacterial Phenomena
Abstract
The results of this project have resulted in several major advances in the development and analysis of genetics systems in the extreme thermophilic archaebacterium. Sulfolobus acidocaldarius. The goal have been to study gene regulation in this group of microbes as essentially nothing gas been done previously due to the lack of tools in these organisms. Methods were developed to routinely culture and plate S. acidocaldarius on solid medium (petri plates). Culture conditions were optimized with respect to medium composition, cell environmental conditions, handling and storage of mutant strains, and development of stable temperature controlled incubators. This organism grows optimally at 75-80 degrees C at a pH of 5-6. Commercially available drying ovens were modified to grow cells on plates at temperatures from 75-78 degrees. Cells can not routinely be plated at high efficiency (ca 90%) on any of a number of standardized plating media types. These include a minimal medium containing a mineral salts mixture plus any of several single carbon supplies as well as rich media.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 28, 1994
- Accession Number
- ADA286106
Entities
People
- Robert P. Gunsalus
Organizations
- University of California, Los Angeles