Biosensors Based on Cross-Linking of Biotinylated Glucose Oxidase By Avidin.

Abstract

Avidin, having four biotin binding units, cross-links and immobilizes glucose oxidase (GOX) labeled with multiple biotins (B-GOX) when solutions of B-GOX and avidin are mixed on an electrode. The H2O2 flux generated in the B-GOX catalyzed oxidation of glucose by dissolved O2 is measured as an electroreduction current at a horseradish peroxidase (HRP) redox conducting hydrogel electrode poised at +100 mV (Ag/AgCl). The sensitivity of the resulting glucose sensor is 0.14A/sq cm/M with a linear range up to 2mM glucose. (jg)

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Document Details

Document Type
Technical Report
Publication Date
Jan 19, 1995
Accession Number
ADA290439

Entities

People

  • Mark S. Vreeke
  • Patrick Rocca

Organizations

  • University of Texas at Austin

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Alcohols
  • Biosensors
  • Chemical Engineering
  • Chemical Synthesis
  • Chemistry
  • Detection
  • Detectors
  • Electrodes
  • Engineering
  • Films
  • Hydrogels
  • New York
  • Oxidation
  • Polymeric Films
  • Polymers
  • Rocket Oxidizers
  • Sensitivity

Fields of Study

  • Chemistry

Readers

  • Electrochemical Surface Science
  • Molecular and Cellular Biochemistry

Technology Areas

  • Biotechnology