Colocalization of Calcium Entry and Exocytotic Release Sites in Adrenal Chromaffin Cells.

Abstract

"Snapshot" images of localized Ca2+ influx into patch-clamped chromaffin cells were captured using a recently developed pulsed-laser imaging system. Transient opening of voltage-sensitive Ca2+ channels gave rise to localized elevations of Ca that had the appearance of either "hotspots" or partial rings found immediately beneath the plasma membrane. When the Ca2+ imaging technique was employed in conjunction with flame-etched carbon-fiber electrodes to spatially map the release sites of catecholamines, it was observed that the sites of Ca2+ entry and catecholamine release were co-localized. These results provide functional support for the idea that secretion occurs from "active zone"-like structures in neuroendocrine cells.

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Document Details

Document Type
Technical Report
Publication Date
Jan 13, 1995
Accession Number
ADA290637

Entities

People

  • Iain M. Robinson
  • Jennefer M. Finnegan
  • Jonathan R. Monck
  • Julio M. Fernandez
  • R. M. Wightman

Organizations

  • University of North Carolina at Chapel Hill

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biological Sciences
  • Blood
  • Carbon Fibers
  • Catecholamines
  • Cell Membrane
  • Cells
  • Cells (Biology)
  • Cellular Structures
  • Chemical Synthesis
  • Chemistry
  • Detection
  • Electrodes
  • Endocrine Cells
  • Endocrine System
  • Imaging Techniques
  • Lasers
  • Secretion

Readers

  • Computer Vision.
  • Electrochemical Surface Science
  • Neuroscience

Technology Areas

  • Directed Energy