Developmental Toxicity (Dominant Lethal Mutation) Study on Agent Lewisite. Dominant Lethal Study of Lewisite in Male Rats.
Abstract
Lewisite (dichloro(2-chlorovinyl)arsine, Agent L) was investigated as part of the US Army Toxicological Program on Chemical Agents. The study was conducted during January - April, 1990. Dosing was performed during 3-12 January, 1990. Twenty male CD rats per dose group were given 1.5, 0.75 or 0.375 mg/kg Lewisite or vehicle control (one ml sesame seed oil) daily by gavage for 5 days. Positive control males were given one ml sesame seed oil by gavage on Day 1-4 and on Day 5 they were given an intraperitoneal injection of 100 mg/kg ethyl methanesulphonate, a known mutagen. Each male was mated to two virgin females (12 weeks of age) per counted and the uteri and contents were examined. Implantation sites were categorized as live/dead fetuses or early/late resorption. No significant differences inreproductive indices were seen between treatment groups and the control group with the exception of the positive control. Males were killed during Week 13 and necropsied. Sperm morphology/modify, testicular histopathologic evaluation and morphometric analysis of seminiferous tubule cross-sections revealed no differences among Lewisite-treated rats and rats given sesame seed oil. There was no indication of a dominant lethal mutagenic or other toxic effect on the male reproductive tract as a result of exposure to Lewisite, under the conditions of this study. The No Observable Adverse Effect Level was the highest dose used, 1.500 mg/kg.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1993
- Accession Number
- ADA290671
Entities
People
- Jack C. Dacre
- Kevin H. Denny
- Robert M. Parker
- Thomas J. Bucci
Organizations
- National Center for Toxicological Research