AFRRI Reports, First Quarter 1995, January - March 1995.

Abstract

This volume contains AFRRI Scientific Reports SR95-1 through SR95-10 for January-March 1995. Cytogenetic detection of kinetochore proteins using the CREST antibody coupled with secondary antibodies labeled with different fluorescent probes has been optimized for several in vitro mammalian cell lines. This study investigated selected parameters including the influence of common fixatives (methanol, ethanol, methanol : acetic acid (3: 1)), detergents (Triton-XlOO, Tween), fluorescent probes (CY3, BODIPY, FITC), washing protocols, and an antifading agent (paraphenylenediamine) on the detection of kinetochore proteins in control and X-ray (240 kVp)-irradiated cells. Utilizing an optimized fixation and staining protocol, a brilliant visualization of kinetochores in interphase cells was obtained in control as well as X-ray-irradiated interhase cells. Application of this improved kinetochore staining methodology readily permits discriminating cells containing either single or paired kineto- chores, the latter of which are characteristic of late-G2 phase and prophase cells.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1995
Accession Number
ADA293794

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  • Armed Forces Radiobiology Research Institute

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  • Cells
  • Chemical Synthesis
  • Chemistry
  • Health Services
  • Medical Personnel
  • Organic Chemistry

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