Cellular Approaches to the Treatment of Clinical Shock.

Abstract

A convenient model system has been developed to examine the effects of energy depletion on cells as a model of clinical shock. Cells are grown on microporous polycarbonate transwells and placed into proximity to a pH sensing silicon chip. Cellular proton flux is recorded as a function of time (extracellular acidification rate). Four cell lines were examined for their suitability in this model system: (1) liver cells; (2) kidney cells; (3) intestinal cells; and (4) tumor cells. Changes in proton flux correlated with other standard viability indices. Further work is being performed to characterize more fully this system.

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Document Details

Document Type
Technical Report
Publication Date
Apr 15, 1995
Accession Number
ADA294162

Entities

People

  • Guy Miller

Tags

Communities of Interest

  • Materials and Manufacturing Processes
  • Sensors
  • Weapons Technologies

DTIC Thesaurus Topics

  • Arteries
  • Cell Line
  • Cells
  • Contracts
  • Culture Techniques
  • Epithelial Cells
  • Glucose
  • Glycolysis
  • Health Services
  • Hemorrhagic Shock
  • Inhibition
  • Payload
  • Phosphorylation
  • Recovery
  • Shock Trauma Centers
  • Standards
  • Therapy

Fields of Study

  • Biology

Readers

  • Computational Modeling and Simulation
  • Electrochemical Engineering/ Fuel Cell Technologies
  • Molecular and Cellular Biology