Chemical Blistering: Cellular and Macromolecular Components.
Abstract
The mission of this project was to determine the cellular and molecular lesions associated with cutaneous vesication from bis(2-chloroethyl)sulfide (BCES). Cultures of keratinocytes were used to focus attention on the direct interactions between the mustard and its epidermal targets. The technical objectives included confirming that DNA was the primary molecular target of BCES in human epidermal keratinocytes, identifying and quantifying BCES- mediated DNA-adducts in relation to dose, determining why epidermal basal cells are more susceptible to BCES than differentiated cells, and investigating the possible role of informational error in DNA in the cytopathogenic process. Data generated in the project suggest that (a) DNA is the primary epidermal target of BCES, and the fidelity of DNA repair governs survival of the germinative population, and (b)BCES causes a decrease in the germinative population by cellular differentiation, as indicated by the appearance of mature keratin protein, as well as necrosis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 31, 1994
- Accession Number
- ADA294612
Entities
People
- I. A. Bernstein
Organizations
- University of Michigan