Munitions Cytotoxicities In Vitro (Animal Test Alternatives)
Abstract
Rapid, inexpensive, and reliable methods are needed for the toxicological screening of chemical substances with the potential to affect health and the environment. One such method, the neutral red (NR) cytotoxicity assay, is based on incorporation of the supravital dye neutral red into lysosomes of viable cells. The NR uptake assay can be used to detect cytotoxic or cytostatic effects of chemical substances capable of damaging cells, has been adapted to microtiter tissue culture systems, and can be analyzed by means of automated spectrophotometric microplate readers (1,2). Neutral red cytotoxicity assays in continuous rat hepatoma H4IIE cells have been applied to a variety of environmentally important munitions and related compounds. The H4IIE cells maintain inducible oxidative microsomal enzymes and were chosen because of their application to detecting other xenobiotics in environmental and biological specimens (6). pg2.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1995
- Accession Number
- ADA294804
Entities
People
- D. R. Wellington
- Harry Salem
- L. M. Dasko-vincent
- W. R. Mitchell
Organizations
- United States Army Biological Warfare Laboratories