Observations of a Circular, Triple-Helical Polysaccharide using Non-Contact Atomic Force Microscopy.
Abstract
The non-contact atomic force microscopy (NC-AFM) technique is applied to achieve molecular resolution of the immunostimulatory (1->8)-branched b(1->3)glucan scleroglucan. Sample preparation involves deposition of the polysaccharide molecules from an aqueous solution onto a freshly cleaved and atomically smooth mica surface by spraying and air drying. High resolution NC-AFM images have been obtained in air from which the mean polymer chain length (and molar mass) and the mean chain diameter have been estimated from measurements on a sizable sample of molecules. jg p.2
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 30, 1995
- Accession Number
- ADA296837
Entities
People
- David A. Brant
- Reginald M. Penner
- Theresa M. Mcintire
Organizations
- University of California, Irvine