The Effects of Signaling Through the EGF Receptor System Upon Regulation of Growth in Human Mammary Epithelial Cells.

Abstract

The levels of the Human EGF Receptor (HER1) and the related receptors HER2,3, and 4 were examined in human-mammary epithelial cells (HMEC's). When HMEC's are grown on plastic tissue culture dishes HER11 is expressed at high levels, while HER2 and HER4 are only expressed at low levels. No HER3 was detected. The expression of the various ligands to these receptors was examined. HMEC's express high levels of amphiregulin; moderate levels of TGF-alpha, HB EGF; and very low level of EGF and B celluin. The effects of HER1 stimulation on the levels of HER2 were examined in both IlMEC's and mouse fibroblasts. Addition of exogenous EGF leads to a decrease in the levels of HER2. This down regulation occurs in 3 hours, this, the mechanism appears to be post transcriptional

Open PDF

Document Details

Document Type
Technical Report
Publication Date
May 31, 1995
Accession Number
ADA297172

Entities

People

  • Becky Worthylake

Organizations

  • University of Utah

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Animals
  • Basements
  • Breast Cancer
  • Cell Line
  • Cells
  • Epithelial Cells
  • Fibroblasts
  • Laboratory Animals
  • Mammary Glands
  • Materials
  • Membranes
  • Neoplasms
  • Recombinant Dna
  • Regulations
  • Standards
  • Tissue Culture

Fields of Study

  • Chemistry

Readers

  • Cardiovascular Physiology
  • Molecular Biology and Genetics