The Role of Gap-Junctional Communication in Chemical Toxicology.
Abstract
The overall goal of this research project was to study the mechanisms by which non-genotoxic chemicals induced multiple disease end points such as birth defects, tumor promotion, reproductive- and neuro-toxicities. The working hypothesis was that these non-genotoxic chemicals disrupted homeostatic control of cell proliferation, differentiation and adaptive responses of differentiated cells. Specifically, to test this hypothesis, gap junctional intercellular communication (GJIC) was studied. During this grant period, several techniques were developed and applied to study how various model non-genotoxic chemicals, as well as various oncogenes, interfered with GJIC to cause abnormal cell growth and differentiation. The molecular biology, biochemistry and cell biology of non-genotoxic chemical and oncogene interference with GJIC was studied using in vitro techniques, primarily with fluorescent detection of ions and molecules via laser-assisted image analyses. jg p.1
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 17, 1995
- Accession Number
- ADA298070
Entities
People
- James E. Trosko
Organizations
- Michigan State University