Isolation and Preliminary Characterization of a Recombinant TAT Protein From Human Immunodeficiency Virus.
Abstract
Human immunodeficiency virus type-1 (HIV-1) Tat protein is a transactivator of viral gene expression. Tat as an over-expressed fusion protein, Rop-Tat has been purified after cyanogen bromide cleavage by using S-Sepharose Q-Sepharose column chromatography and subsequent use of HPLC. It is about 90% pure and the major impurity is a peptide which is 4 amino acids shorter than Tat, caused by breaking the acid labile bond of aspartate-proline under the acidic cleavage condition of cyanogen bromide. The fusion protein, Rop-Tat was purified by using the same procedures used for Tat. The next step will be refolding of Tat and Rop-Tat.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 23, 1995
- Accession Number
- ADA298304
Entities
People
- Lichun H. Walls
Organizations
- Morgan State University